Real-time tracking of fast axonal transport of acidic vesicles in live adult mouse dorsal root ganglion (DRG) and cortical neurons in brain slices was visualized by the use of binuclear cycloplatinated complexes, {[Pt(L-1)](2)(mu-dppm)}(2+) (1) and {[Pt(L-2)](2)(mu-dppm)}(2+) (2), as lysosome-specific two-photon imaging probes.
This work was supported in part by ECS/GRF grants from The Research Grant Council of the Hong Kong Special Administrative Region Government (9041748, 9041948), Centre for Biosystems, Neuroscience, and Nanotechnology at City University of Hong Kong and The City University of Hong Kong Startup Fund (7200263) and Seed Funding (7003028) award to Chi Ma. W.-Y. W. acknowledges the financial support from the Hong Kong Research Grants Council (HKBU202410 and HKUST2/CRF/10) and the National Basic Research Program of China (973 Program) (2013CB834702).