Abstract
Shift work is associated with insulin resistance, dyslipidaemia and metabolic syndrome, but how consecutive meals at the same clock times differentially shape postprandial metabolism in day-and night-shifts remains unclear. This study used a unique simulated shift work intervention with four consecutive meals and high-resolution sampling to examine interactions of endoge-nous rhythms and behavioural timing on postprandial metabolism. Nine healthy non-shift-worker participants (age 21–33 years, 3 females) completed a strictly-controlled laboratory protocol with a day-shift followed by two night-shifts of 10-h delayed sleep and mealtimes. Participants consumed four identical, isoenergetic, mixed-composition meals per shift. Three of four mealtimes (08:00, 18:00, 22:00) were fixed across conditions; the first and last meals were 1 h after waking and before bedtime. Plasma glucose, insulin, triglycerides, HDL-cholesterol and total cholesterol were measured hourly, with continuous interstitial glucose monitoring. Subjective hunger and appetite were assessed throughout. Plasma lipid rhythms shifted with the 10-h delay in sleep– wake times: acrophases delayed ~10–13 h and amplitudes lowered 46%–54% (all p < 0.01). Night-shifts elevated postprandial glucose (+165 mmol/L·min, 95% CI 1.05–34.31, p = 0.05) and insulin (+7678.7 mmol/L·min, 95% CI 3671.02–11686.38, p < 0.01). Time-of-day effects persisted irrespective of shift, with 18:00 meals producing the highest glycaemic responses (+261 mmol/ L·min, 95% CI 124.37–398.01, p < 0.01). Insulin dynamics did not fully compensate for elevated glycaemia in night-shifts, indicating lower glucose tolerance and insulin sensitivity. Mistimed meals suggest a mechanism by which circadian disruption may increase metabolic disease risk.