Abstract
High‐titre, high‐specificity antisera against diacetoxyscirpenol have been produced in rabbits and used to set up an indirect, microtitration‐plate enzyme‐linked immunosorbent assay for the trichothecene mycotoxin. The assay, with a limit of detection of 2 pg per well, has been applied to the determination of diacetoxyscirpenol in wheat. Cereal extraction consists of a simple solubilisation of toxin in methanol resulting in an assay sensitivity of 300 ng g−1. Recovery, measured at three different toxin levels, was essentially quantitative.