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Phenotypic investigation of mononuclear phagocytes by flow cytometry
Journal article   Peer reviewed

Phenotypic investigation of mononuclear phagocytes by flow cytometry

F. Steinbach and B. Thiele
Journal of immunological methods, Vol.174(1), pp.109-122
14/09/1994
PMID: 8083514

Abstract

Dendritic cell Differentiation Equine Intracellular antigen Monocyte Flow Cytometry Macrophage
A simple and rapid method for the phenotypic analysis of mononuclear phagocytes is described. Using flow cytometry, surface markers, as well as intracellular antigens and DNA content of peripheral blood monocytes, or differentiated monocytic cells could be analysed. Furthermore, the method has been of use in several species and is therefore not restricted to applications in humans. Different modes of data aquisition and analysis were compared and are discussed.

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