Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

Francesca Genova; Simona Nonnis; Elisa Maffioli; Gabriella Tedeschi; Maria Giuseppina Strillacci; Michela Carisetti; Giuseppe Sironi; Francesca Anna Cupaioli; Noemi Di Nanni; Alessandra Mezzelani; Ettore Mosca; Christopher R. Helps; Peter A. J. Leegwater; Laetitia Dorso; Maria Longeri; Reuben M. Buckley; Danielle Aberdein; Paulo C. Alves; Asa Ohlsson Andersson; Gregory S. Barsh; Rebecca R. Bellone; Tomas F. Bergstrom; Adam R. Boyko; Jeffrey A. Brockman; Margret L. Casal; Marta G. Castelhano; Ottmar Distl; Nicholas H. Dodman; N. Matthew Ellinwood; Jonathan E. Fogle; Oliver P. Forman; Dorian J. Garrick; Edward Ginns; Bianca Haase; Jens Haggstrom; Robert J. Harvey; Daisuke Hasegawa; Isabel Hernandez; Marjo K. Hytonen; Maria Kaukonen; Christopher B. Kaelin; Tomoki Kosho; Emilie Leclerc; Teri L. Lear; Tosso Leeb; Ronald H. L. Li; Hannes Lohi; Mark A. Magnuson; Richard Malik; Shrinivasrao P. Mane; John S. Munday; William J. Murphy; Niels C. Pedersen; Simon M. Peterson-Jones; Max F. Rothschild; Clare Rusbridge; Beth Shapiro; Joshua A. Stern; William F. Swanson; Karen A. Terio; Rory J. Todhunter; Wesley C. Warren; Elizabeth A. Wilcox; Julia H. Wildschutte; Yoshihiko Yu; Leslie A. Lyons; 99 Lives Consortium

doi:10.1038/s41598-021-87168-0

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Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

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Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

Francesca Genova, Simona Nonnis, Elisa Maffioli, Gabriella Tedeschi, Maria Giuseppina Strillacci, Michela Carisetti, Giuseppe Sironi, Francesca Anna Cupaioli, Noemi Di Nanni, Alessandra Mezzelani, …

Scientific reports, Vol.11(1), 8339

16/04/2021

DOI: https://doi.org/10.1038/s41598-021-87168-0

PMID: 33863921

Abstract

Multidisciplinary Sciences

Science & Technology

Science & Technology - Other Topics

The amyloidoses constitute a group of diseases occurring in humans and animals that are characterized by abnormal deposits of aggregated proteins in organs, affecting their structure and function. In the Abyssinian cat breed, a familial form of renal amyloidosis has been described. In this study, multi-omics analyses were applied and integrated to explore some aspects of the unknown pathogenetic processes in cats. Whole-genome sequences of two affected Abyssinians and 195 controls of other breeds (part of the 99 Lives initiative) were screened to prioritize potential disease-associated variants. Proteome and miRNAome from formalin-fixed paraffin-embedded kidney specimens of fully necropsied Abyssinian cats, three affected and three non-amyloidosis-affected were characterized. While the trigger of the disorder remains unclear, overall, (i) 35,960 genomic variants were detected; (ii) 215 and 56 proteins were identified as exclusive or overexpressed in the affected and control kidneys, respectively; (iii) 60 miRNAs were differentially expressed, 20 of which are newly described. With omics data integration, the general conclusions are: (i) the familial amyloid renal form in Abyssinians is not a simple monogenic trait; (ii) amyloid deposition is not triggered by mutated amyloidogenic proteins but is a mix of proteins codified by wild-type genes; (iii) the form is biochemically classifiable as AA amyloidosis.

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Title: Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits
Creators: Francesca Genova - University of Milan
Simona Nonnis - University of Milan
Elisa Maffioli - University of Milan
Gabriella Tedeschi - University of Milan
Maria Giuseppina Strillacci - University of Milan
Michela Carisetti - University of Milan
Giuseppe Sironi - University of Milan
Francesca Anna Cupaioli - Institute of Biomedical Technologies
Noemi Di Nanni - Institute of Biomedical Technologies
Alessandra Mezzelani - Institute of Biomedical Technologies
Ettore Mosca - Institute of Biomedical Technologies
Christopher R. Helps - University of Bristol
Peter A. J. Leegwater - Utrecht University
Laetitia Dorso - Oniris
Maria Longeri - University of Milan
Reuben M. Buckley - University of Missouri
Danielle Aberdein - Massey University
Paulo C. Alves - Universidade do Porto
Asa Ohlsson Andersson - College Station Medical Center
Gregory S. Barsh - HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA
Rebecca R. Bellone - Univ Calif Davis, Vet Genet Lab, Davis, CA 95616 USA
Tomas F. Bergstrom - Swedish Univ Agr Sci, Dept Anim Breeding & Genet, S-75007 Uppsala, Sweden
Adam R. Boyko - Cornell University
Jeffrey A. Brockman - Hills Pet Nutr Inc, Topeka, KS 66601 USA
Margret L. Casal - Supreme Council Of Health
Marta G. Castelhano - Cornell University
Ottmar Distl - College Station Medical Center
Nicholas H. Dodman - Tufts University
N. Matthew Ellinwood - Natl MPS Soc, POB 14696, Durham, NC 27709 USA
Jonathan E. Fogle - North Carolina State University
Oliver P. Forman - WALTHAM Ctr Pet Nutr, Freeby Lane, Melton Mowbray LE14 4RT, Leics, England
Dorian J. Garrick - Massey University
Edward Ginns - Supreme Council Of Health
Bianca Haase - University of Sydney
Jens Haggstrom - College Station Medical Center
Robert J. Harvey - University of the Sunshine Coast
Daisuke Hasegawa - Nippon Vet & Life Sci Univ, Lab Vet Radiol, Musashino, Tokyo 1808602, Japan
Isabel Hernandez - Cornell University
Marjo K. Hytonen - University of Helsinki
Maria Kaukonen - University of Helsinki
Christopher B. Kaelin - HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA
Tomoki Kosho - Shinshu Univ, Dept Med Genet, Sch Med, Matsumoto, Nagano 3908621, Japan
Emilie Leclerc - Symrise
Teri L. Lear - University of Kentucky
Tosso Leeb - University of Bern
Ronald H. L. Li - Supreme Council Of Health
Hannes Lohi - University of Helsinki
Mark A. Magnuson - Vanderbilt University
Richard Malik - University of Sydney
Shrinivasrao P. Mane - Elanco
John S. Munday - Massey University
William J. Murphy - Texas A&M Univ, Coll Vet Med, Dept Vet Integrat Biosci, College Stn, TX 77845 USA
Niels C. Pedersen - Supreme Council Of Health
Simon M. Peterson-Jones - Michigan State University
Max F. Rothschild - Iowa State University
Clare Rusbridge - Univ Surrey, Fac Hlth & Med Sci, Sch Vet Med, Guildford GU2 7AL, Surrey, England
Beth Shapiro - Univ Calif Santa Cruz, Dept Ecol & Evolutionary Biol, Santa Cruz, CA 95064 USA
Joshua A. Stern - Supreme Council Of Health
William F. Swanson - Cincinnati Zoo & Bot Garden, Ctr Conservat & Res Endangered Wildlife CREW, Cincinnati, OH 45220 USA
Karen A. Terio - Univ Illinois, Zool Pathol Program, Brookfield, IL 60513 USA
Rory J. Todhunter - Cornell University
Wesley C. Warren - University of Missouri
Elizabeth A. Wilcox - Cornell University
Julia H. Wildschutte - Bowling Green State University
Yoshihiko Yu - Nippon Vet & Life Sci Univ, Lab Vet Radiol, Musashino, Tokyo 1808602, Japan
Leslie A. Lyons - University of Missouri
99 Lives Consortium
Publication Details: Scientific reports, Vol.11(1), 8339
Publisher: NATURE PORTFOLIO
Number of pages: 14
Date published: 16/04/2021
Grant note: 686 / Universita degli Studi di Milano -Italy W16-028; W18-040 / Winn Feline Foundation ANFI (Associazione Nazionale Felina Italiana)
Identifiers: 99788139802346
Academic Unit: School of Veterinary Medicine
Language: English
Resource Type: Journal article

Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

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