Abstract
The feasibility of municipal sewage treatment in laboratory-scale up-flow anaerobic sludge blankets was investigated in this work. Unlike previous studies, granular activated carbon (conductive) or sponge (non-conductive) was introduced to hollow plastic balls as carriers and suspended in the middle and upper layers of the reactors. The two bioreactors were operated at four different hydraulic retention times (stepwise reduced from 24 h to 8 h) for 100 days at ∼18 °C. The conductive-amended treatment was more effective than the non-conductive treatment in enhancing reactor performance. Interestingly, in the reactor containing conductive carriers, microorganisms enriched in the conductive biofilm were also dominant in the suspended sludge. In the reactor containing sponge carriers, the dominant microorganisms differed between the non-conductive biofilm and the suspended sludge. This study underlines that the enrichment of functional microbial communities and the positive impacts of biofilm on suspended sludge are the keys to improving biomethane recovery.