Immunogenicity profiling of protein antigens from capsular group B Neisseria meningitidis

Amaka M Awanye; Chun-Mien Chang; Jun X Wheeler; Hannah Chan; Leanne Marsay; Christina Dold; Christine S. Rollier; Louise E Bird; Joanne E Nettleship; Raymond J Owens; Andrew J. Pollard; Jeremy P Derrick

doi:10.1038/s41598-019-43139-0

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Immunogenicity profiling of protein antigens from capsular group B Neisseria meningitidis

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Immunogenicity profiling of protein antigens from capsular group B Neisseria meningitidis

Amaka M Awanye, Chun-Mien Chang, Jun X Wheeler, Hannah Chan, Leanne Marsay, Christina Dold, Christine S. Rollier, Louise E Bird, Joanne E Nettleship, Raymond J Owens, …

Scientific Reports, Vol.9(1), pp.6843-6843

02/05/2019

DOI: https://doi.org/10.1038/s41598-019-43139-0

PMCID: PMC6497663

PMID: 31048732

Abstract

Adolescent

Adult

Animals

Antigens, Bacterial - immunology

Bacterial Vaccines - immunology

Bacterial Vaccines - therapeutic use

Chromatography, Gel

Female

Humans

Immunoglobulin G - immunology

Male

Mice

Mice, Inbred C57BL

Neisseria meningitidis, Serogroup B - immunology

Porins - immunology

Porins - metabolism

Young Adult

Mass Spectrometry

Outer membrane vesicle (OMV)- based vaccines have been used to provide strain-specific protection against capsular group B Neisseria meningitidis infections, but the full breadth of the immune response against the components of the OMV has not been established. Sera from adults vaccinated with an OMV vaccine were used to screen 91 outer membrane proteins (OMPs) incorporated in an antigen microarray panel. Antigen-specific IgG levels were quantified pre-vaccination, and after 12 and 18 weeks. These results were compared with IgG levels from mice vaccinated with the same OMV vaccine. The repertoires of highly responding antigens in humans and mice overlapped, but were not identical. The highest responding antigens to human IgG comprised four integral OMPs (PorA, PorB, OpcA and PilQ), a protein which promotes the stability of PorA and PorB (RmpM) and two lipoproteins (BamC and GNA1162). These observations will assist in evaluating the role of minor antigen components within OMVs in providing protection against meningococcal infection. In addition, the relative dominance of responses to integral OMPs in humans emphasizes the importance of this subclass and points to the value of maintaining conformational epitopes from integral membrane proteins in vaccine formulations.

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Title: Immunogenicity profiling of protein antigens from capsular group B Neisseria meningitidis
Creators: Amaka M Awanye - Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, M13 9PL, UK
Chun-Mien Chang - Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, M13 9PL, UK
Jun X Wheeler - National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Hertfordshire, EN6 3QG, UK
Hannah Chan - National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Hertfordshire, EN6 3QG, UK
Leanne Marsay - Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, OX3 7LE, UK
Christina Dold - Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, OX3 7LE, UK
Christine S. Rollier
Louise E Bird - Oxford Protein Production Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell, Didcot, OX11 0FA, UK
Joanne E Nettleship - Oxford Protein Production Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell, Didcot, OX11 0FA, UK
Raymond J Owens - Oxford Protein Production Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell, Didcot, OX11 0FA, UK
Andrew J. Pollard - Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, OX3 7LE, UK
Jeremy P Derrick - Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, M13 9PL, UK. Jeremy.Derrick@manchester.ac.uk
Publication Details: Scientific Reports, Vol.9(1), pp.6843-6843
Publisher: Nature Research
Date published: 02/05/2019
Date accepted: 11/04/2019
Grants: MR/K018779/1, Medical Research Council (United Kingdom, London) - MRC
Grant note: A.M.A. was the recipient of TETFund (Nigeria), while C.M.C. was a recipient of Taiwan Bio-Development Foundation and The MOE (Taiwan) Technologies Incubation Scholarship. The MenPF-1 Phase I trial was funded by a Wellcome Trust Strategic Translation Award (Award number: 082102/Z/07/A). RJO LEB and JEN acknowledge support from the UK Medical Research Council (MR/K018779/1). We thank Angela Thistlethwaite for laboratory assistance and Saloni Sonawala (Arrayjet Ltd) for her help with the microarray printing. CR and AJP are Jenner investigators.
Identifiers: 99616365702346
Copyright: Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Academic Unit: School of Biosciences
Language: English
Resource Type: Journal article

Immunogenicity profiling of protein antigens from capsular group B Neisseria meningitidis

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