Abstract
Pseudomonas aeruginosa is a prevalent healthcare pathogen. It forms biofilms, which significantly increase antibiotic resistance, escalating the burden on healthcare systems. While global biofilm phenomena are observable experimentally, understanding local behaviours of bacteria and their environment, and how these factors drive biofilm formation, is challenging. Agent-based modelling (ABM) offers a computational solution to simulate these local behaviours and resulting large-scale phenomena. ABMs can simulate physical mechanisms like erosion and sloughing but often lack the ability to capture them during biofilm growth and validate them experimentally. We present a three-dimensional ABM of P. aeruginosa biofilms growing under constant flow. This model is the first of its kind to accurately capture the change in physical shape of biofilms growing under constant flow, as well as capture erosion and sloughing as natural byproducts of local bacteria interactions. The model is validated both qualitatively and quantitatively against experimental culture results, providing a general framework that can be adapted to simulate different environments or other biofilm-forming species.