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The Acute Effect of Consuming Whey Versus a Plant‐Based Protein Blend on Postprandial Metabolism and Appetite in a Sample of Healthy Adults
Journal article   Open access   Peer reviewed

The Acute Effect of Consuming Whey Versus a Plant‐Based Protein Blend on Postprandial Metabolism and Appetite in a Sample of Healthy Adults

Food science & nutrition, Vol.14(2), e71485
02/2026
PMID: 41695112

Abstract

Original Nutrition or Dietetics
There is controversy regarding the differential metabolic effects of proteins from plant and animal sources. Existing literature focuses on a limited range of plant proteins consumed just before, or as part of, a mixed meal, resulting in limited insight into the differential effects of plant proteins when ingested alone. This study compared the acute effects of a mixed plant‐based protein supplement and whey, when ingested in a fasted state and in the absence of other nutrients, on postprandial metabolism, appetite, and subsequent energy intake. Fifteen healthy adults completed three study visits in a randomized single blind crossover study design. Participants consumed 20 g protein from whey or a plant‐based protein supplement (blend of pea, brown rice, pumpkin seeds) mixed in water, or an equal volume of water as control. Plasma ketones, non‐esterified fatty acids (NEFA), glucose, insulin, and glucagon‐like peptide 1 (GLP‐1) were measured over 3 h postprandial, alongside resting energy expenditure (REE), respiratory exchange ratio (RER), and perceived appetite. Intake from an ad libitum lunch meal was used to assess subsequent energy consumption. There was a transient reduction in postprandial ketones and NEFA, and elevation of insulin and GLP‐1 following protein ingestion. Compared to plant protein, whey resulted in lower NEFA at T30 (p = 0.037) and higher insulin concentrations at T30 (p < 0.001) and T60 (p = 0.010). REE, RER, subjective appetite ratings and subsequent energy intake were not different between the two proteins, relative to the control. The results from this study indicate that whey and a plant protein blend supplement have comparable metabolic effects. This study compares the metabolic effects of whey and a plant protein blend, consumed in a fasted state. The results showed a transient reduction in postprandial ketones and NEFA, and elevation of insulin and GLP‐1 following protein ingestion. The two protein drinks exerted a comparable effect on resting energy expenditure, subjective appetite ratings, and subsequent energy intake, and did not affect respiratory exchange ratio —indicating that whey and a plant protein blend supplement have comparable metabolic effects.
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https://doi.org/10.1002/fsn3.71485View
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