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Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
Journal article   Open access  Peer reviewed

Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

S Piluso, HC Cassell, JL Gibbons, TE Waller, NJ Plant, AF Miller and G Cavalli
Soft Matter, Vol.9(29), pp.6752-6756
07/08/2013

Abstract

The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.
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http://dx.doi.org/10.1039/c3sm00131hView
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http://www.ncbi.nlm.nih.gov/pubmed/23847687View
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