Simple enzymic method for isolation of DNA from diverse bacteria

Shico Visuvanathan; Michael T. Moss; John L. Stanford; John Hermon-Taylor; John J. McFadden; Johnjoe Mcfadden

doi:10.1016/0167-7012(89)90001-8

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Simple enzymic method for isolation of DNA from diverse bacteria

Journal article

Peer reviewed

Simple enzymic method for isolation of DNA from diverse bacteria

Shico Visuvanathan, Michael T. Moss, John L. Stanford, John Hermon-Taylor, John J. McFadden and Johnjoe Mcfadden

Journal of microbiological methods, Vol.10(2), pp.59-64

01/09/1989

DOI: https://doi.org/10.1016/0167-7012(89)90001-8

Abstract

Bacterium

DNA extraction

Enzymic

An enzymic method for the extraction of high molecular weight DNA from a wide range of diverse bacteria, including mycobacteria, is described. The method is simple, rapid, requires few manipulations and is suitable for extracting quantitative yields of DNA from as few as ≈3×10 7 bacterial cells. The DNA produced is of good quality and is suitable for molecular biological manipulations.

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Title: Simple enzymic method for isolation of DNA from diverse bacteria
Creators: Shico Visuvanathan - Middlesex University
Michael T. Moss - St George's Hospital
John L. Stanford - Department of Medical Microbiology, University College and Middlesex School of Medicine, London, UK
John Hermon-Taylor - St George's Hospital
John J. McFadden - St George's Hospital
Johnjoe Mcfadden - School of Biosciences and Medicine
Publication Details: Journal of microbiological methods, Vol.10(2), pp.59-64
Publisher: Elsevier B.V
Date published: 01/09/1989
Identifiers: 99783259002346
Academic Unit: School of Biosciences
Language: English
Resource Type: Journal article

Simple enzymic method for isolation of DNA from diverse bacteria

Abstract

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