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Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
Journal article

Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.

SE Eckert, F Dziva, RR Chaudhuri, GC Langridge, DJ Turner, DJ Pickard, DJ Maskell, NR Thomson and MP Stevens
J Bacteriol, Vol.193(7), pp.1771-1776
04/2011
DOI: https://doi.org/10.1128/JB.01292-10

Abstract

Animals Cattle Cattle Diseases Chromosomes Bacterial DNA Transposable Elements DNA Bacterial Escherichia coli O157 Genetic Fitness Genotype Mutagenesis Insertional Plasmids
Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
url
http://www.ncbi.nlm.nih.gov/pubmed/21278291View
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