RNA-based spatial community analysis revealed intra-reactor variation and expanded collection of direct interspecies electron transfer microorganisms in anaerobic digestion

Bing Guo; Yingdi Zhang; Lei Zhang; Yun Zhou; Yang Liu

doi:10.1016/j.biortech.2019.122534

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RNA-based spatial community analysis revealed intra-reactor variation and expanded collection of direct interspecies electron transfer microorganisms in anaerobic digestion

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RNA-based spatial community analysis revealed intra-reactor variation and expanded collection of direct interspecies electron transfer microorganisms in anaerobic digestion

Bing Guo, Yingdi Zhang, Lei Zhang, Yun Zhou and Yang Liu

Bioresource technology, Vol.298, 122534

02/2020

DOI: https://doi.org/10.1016/j.biortech.2019.122534

PMID: 31835200

Abstract

Anaerobiosis

Electrons

Sewage

Bioreactors

Methane

RNA

Granular activated carbon (GAC) has been shown to mediate direct interspecies electron transfer (DIET) in anaerobic digestion. Adding GAC to up-flow anaerobic sludge bed reactor increased the total biomass slightly from 20.0 to 26.6 gVSS/reactor, and maximum organic removal capacity remarkably from 285 to 1660 mgCOD/L/d. Since GAC occupied 7% of reactor volume (denser than suspended sludge, settled to the reactor bottom), we used a spatial sampling strategy (sludge bed top/mid/bottom layers, and tightly attached GAC-biofilm) and DNA- and RNA-based community analyses. RNA-based analysis demonstrated significant community differences between the non-GAC and GAC-amended reactors (p < 0.05) based on ANOSIM statistical analysis. In comparison, DNA-based analysis showed little community difference between these reactors (p > 0.05). RNA-based analysis revealed active enrichments in GAC-biofilm, including bacteria Geobacter, Syntrophus, Desulfovibrio and Blvii28, and archaea Methanosaeta and Methanospirillum. These are potential electro-active syntrophic microorganisms related with DIET, which expand the previously defined list of DIET microorganisms.

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Title: RNA-based spatial community analysis revealed intra-reactor variation and expanded collection of direct interspecies electron transfer microorganisms in anaerobic digestion
Creators: Bing Guo - University of Surrey, School of Sustainability, Civil and Environmental Engineering
Yingdi Zhang - Department of Civil and Environmental Engineering, University of Alberta, T6G 1H9 Edmonton, Canada
Lei Zhang - Department of Civil and Environmental Engineering, University of Alberta, T6G 1H9 Edmonton, Canada
Yun Zhou - Department of Civil and Environmental Engineering, University of Alberta, T6G 1H9 Edmonton, Canada
Yang Liu - University of Surrey, School of Computer Science and Electronic Engineering
Publication Details: Bioresource technology, Vol.298, 122534
Publisher: Elsevier
Date published: 02/2020
Date accepted: 28/11/2019
Grant note: The authors acknowledge the financial support for this project provided by research grants from a Natural Sciences and Engineering Research Council of Canada (NSERC) Strategic Partnership Grants for Projects (SPG-P), an NSERC Industrial Research Chair (IRC) Program in Sustainable Urban Water Development (Liu, Y.) through the support by EPCOR Water Services, EPCOR Drainage Operation, Alberta Innovates, and WaterWerx, and the Canada Research Chair (CRC) in Future Community Water Services (Liu, Y.).
Identifiers: 99606722402346
Academic Unit: School of Sustainability, Civil and Environmental Engineering
Language: English
Resource Type: Journal article

RNA-based spatial community analysis revealed intra-reactor variation and expanded collection of direct interspecies electron transfer microorganisms in anaerobic digestion

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