Abstract
Effect of disaccharides on the stability of lyophilic colloid systems (vesicles of phosphatidylcholine) at low temperatures was studied using 2H-NMR spectroscopy. Two programs of vesicles treatment were used: ′cooling down to -30°C - heating at room temperature′ and ′cooling down to -196°C - drying - rehydration′. It was shown that NMR spectra of treated vesicles totally disappeared indicating the decomposition of bilayer. The presence of disaccharides in vesicles dispersions during their treatment prevented the disappearance of the spectra. Optimum concentrations of disaccharides for the formation of safe layer around the vesicles were calculated. It was suggested that the safe properties of disaccharides were caused by their binding with membrane surface and action to water structure.