Abstract
This report presents the results of an investigation into the application of micellar electrokinetic capillary chromatography (MECC) for the analysis of limonoid glucosides in a citrus seed extract. MECC based on sodium dodecyl sulphate (SDS) was used to provide highly efficient separations of the closely related structures. A phosphate-borate buffer containing SDS was used to optimize the separation conditions for a mixture containing standard compounds of the glucosides of limonin, obacunone and nomilin. The study showed that MECC analysis of limonoid glucosides requires minimal sample clean-up steps and achieves excellent separations within a relatively short analysis time. The technique developed was used to quantify two limonoid glucosides in a methanol extract of the seeds of
Citrus nobilis (nartjie) and
Citrus limon (lemon).