Metagenomic insights into direct interspecies electron transfer and quorum sensing in blackwater anaerobic digestion reactors supplemented with granular activated carbon

Hongyu Dang; Najiaowa Yu; Anqi Mou; Lei Zhang; Bing Guo; Yang Liu

doi:10.1016/j.biortech.2022.127113

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Metagenomic insights into direct interspecies electron transfer and quorum sensing in blackwater anaerobic digestion reactors supplemented with granular activated carbon

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Metagenomic insights into direct interspecies electron transfer and quorum sensing in blackwater anaerobic digestion reactors supplemented with granular activated carbon

Hongyu Dang, Najiaowa Yu, Anqi Mou, Lei Zhang, Bing Guo and Yang Liu

Bioresource technology, Vol.352, 127113

05/2022

DOI: https://doi.org/10.1016/j.biortech.2022.127113

PMID: 35381332

Abstract

Microbial community analysis

Direct interspecies electron transfer (DIET)

Shotgun sequencing

The addition of granular activated carbon (GAC) enhanced the performance of up-flow anaerobic sludge blanket (UASB) reactor treating blackwater at 35 °C. DNA were extracted from the sludge and biofilms attached to GAC and submitted for shotgun sequencing. In addition, the acyl-homoserine lactones (AHLs) were quantified. Diverse partners for direct interspecies electron transfer (DIET) were enriched in the sludge or biofilm (GAC-biofilm) of GAC amended UASB. Pedosphaera parvula, Syntrophus aciditrophicus and Syntrophorhabus aromaticivorans were dominant syntrophs. The analysis for type IV pilus assembly genes further suggested DIET may be functioned through GAC for GAC-biofilm, while through conductive pili for sludge aggregates. AHLs quantification and the analysis for quorum sensing (QS) related genes indicated higher QS activity at the population level was induced by GAC. Overall, the work illustrated the different DIET patterns, and suggested that QS played an important role in controlling the performance in GAC amended USAB.

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Title: Metagenomic insights into direct interspecies electron transfer and quorum sensing in blackwater anaerobic digestion reactors supplemented with granular activated carbon
Creators: Hongyu Dang - Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada
Najiaowa Yu - Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada
Anqi Mou - Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada
Lei Zhang - Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada
Bing Guo
Yang Liu - Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada
Publication Details: Bioresource technology, Vol.352, 127113
Publisher: Elsevier
Date published: 05/2022
Date accepted: 01/04/2022
Grant note: The authors acknowledge the financial support for this project provided by research grants from the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant, an NSERC Strategic Partnership Grant for Projects (SPG-P), the University of Alberta PILOT Seed Grant Program, the Canada Research Chair (CRC) in Future Water Services (Liu, Y.), and the China Scholarship Council (CSC) (Na, J. and Mou, A.).
Identifiers: 99644365002346
Academic Unit: School of Sustainability, Civil and Environmental Engineering
Language: English
Resource Type: Journal article

Metagenomic insights into direct interspecies electron transfer and quorum sensing in blackwater anaerobic digestion reactors supplemented with granular activated carbon

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