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Isolation of Oropouche Virus from Febrile Patient, Ecuador
Journal article   Peer reviewed

Isolation of Oropouche Virus from Febrile Patient, Ecuador

EMMA WISE, Steven T Pullan, Sully Márquez, Verónica Paz, Juan D Mosquera, Sonia Zapata, Simon K Jackson, Gyorgy Fejer, Gabriel Trueba and Christopher H Logue
Emerging infectious diseases, Vol.24(5), pp.935-937
01/05/2018
DOI: https://doi.org/10.3201/eid2405.171569
PMCID: PMC5938787
PMID: 29664378

Abstract

Orthobunyavirus - genetics Humans Cercopithecus aethiops Male Ecuador - epidemiology Phylogeny Reverse Transcriptase Polymerase Chain Reaction Animals Bunyaviridae Infections - virology Adult Orthobunyavirus - isolation & purification Real-Time Polymerase Chain Reaction Vero Cells Bunyaviridae Infections - epidemiology
We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.
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