Investigation of the electrostatic field of the papain active centre by using monoprotonated and diprotonated pyridyl (Py) disulphides as reactivity probes

Sheraz Gul; ANNA Clarke; Benjamin C. T. Field; MARK P. Thomas; FRANCES Willenbrock; SURAPONG Pinitglang; CHANDRA Verma; EMRYS W. Thomas; KEITH Brocklehurst

doi:10.1042/bst025091s

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Investigation of the electrostatic field of the papain active centre by using monoprotonated and diprotonated pyridyl (Py) disulphides as reactivity probes

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Investigation of the electrostatic field of the papain active centre by using monoprotonated and diprotonated pyridyl (Py) disulphides as reactivity probes

Sheraz Gul, ANNA Clarke, Benjamin C. T. Field, MARK P. Thomas, FRANCES Willenbrock, SURAPONG Pinitglang, CHANDRA Verma, EMRYS W. Thomas and KEITH Brocklehurst

Biochemical Society transactions, Vol.25(1), pp.91S-91S

01/02/1997

DOI: https://doi.org/10.1042/bst025091s

PMID: 9056989

Abstract

Stopped-flow spectrophotometry

Cysteine proteinases

Papain

Chemical Kinetics

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Title: Investigation of the electrostatic field of the papain active centre by using monoprotonated and diprotonated pyridyl (Py) disulphides as reactivity probes
Creators: Sheraz Gul (Author) - Laboratory of Structural and Mechanistic Enzymology, Department of Biochemistry, Queen Mary and Westfield College, University of London
ANNA Clarke (Author) - Laboratory of Structural and Mechanistic Enzymology, Department of Biochemistry, Queen Mary and Westfield College, University of London, E1 4NS, UK
Benjamin C. T. Field (Author)
MARK P. Thomas (Author) - Laboratory of Structural and Mechanistic Enzymology, Department of Biochemistry, Queen Mary and Westfield College, University of London, E1 4NS, UK
FRANCES Willenbrock (Author) - Laboratory of Structural and Mechanistic Enzymology, Department of Biochemistry, Queen Mary and Westfield College, University of London, E1 4NS, UK
SURAPONG Pinitglang (Author) - Laboratory of Structural and Mechanistic Enzymology, Department of Biochemistry, Queen Mary and Westfield College, University of London, E1 4NS, UK
CHANDRA Verma (Author) - Protein Structure Research Group, Department of Chemistry, University of York, Heslington, York, YO1 5DD, UK
EMRYS W. Thomas (Author) - Department of Biological Sciences, University of Salford, Salford, M5 4JW, UK
KEITH Brocklehurst (Corresponding Author) - Laboratory of Structural and Mechanistic Enzymology, Department of Biochemistry, Queen Mary and Westfield College, University of London, E1 4NS, UK
Publication Details: Biochemical Society transactions, Vol.25(1), pp.91S-91S
Publisher: Biochemical Society
Date published: 01/02/1997
Identifiers: 99696063202346
Academic Unit: School of Biosciences
Language: English
Resource Type: Journal article

Investigation of the electrostatic field of the papain active centre by using monoprotonated and diprotonated pyridyl (Py) disulphides as reactivity probes

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