Inter-laboratory Validation of Procedures for Measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2′-deoxyguanosine in DNA

Andrew R. Collins; Catherine Gedik; Sharon Wood; Ann White; Jacques Dubois; Pierre Duez; Jean-François Rees; Rozenn Legall; Liliane Degand; Steffen Loft; Annie Jensen; Henrik Poulsen; Allan Weimann; Bente Riis Jensen; Jean Cadet; Thierry Douki; Jean-Luc Ravanat; Henry Faure; Michèle Tripier; Isabelle Morel; Odile Sergent; Pierre Cillard; Bénédicte Morin; Bernd Epe; Nicole Phoa; Andrea Hartwig; Anke Pelzer; Piero Dolara; Chiara Casalini; Francesco Guglielmi; Cristina Luceri; Hiroshi Kasai; Rie Kido; Ryszard Olinski; Karol Bialkowski; Zdena Dura ková; Lucia Hlin iková; Peter Korytar; Mária Dušinská; Csilla Mislanová; José Viña; Ana Lloret; Lennart Möller; Tim Hofer; Eric Gremaud; Laurent Fay; Richard Stadler; Julie Eakins; François Pognan; John O'Brien; Ruan Elliott; Siân Astley; Angela Bailley; Karl Herbert; Dina Chauhan; Frank Kelly; Chrisi Dunster; Joseph Lunec; Ian Podmore; Parul Patel; Samantha Johnson; Mark Evans; Andrea White; Rex Tyrrell; Matthew Gordon; Chris Wild; Laura Hardie; Elizabeth Smith; ESCODD

doi:10.1080/10715760290019246

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Inter-laboratory Validation of Procedures for Measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2′-deoxyguanosine in DNA

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Inter-laboratory Validation of Procedures for Measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2′-deoxyguanosine in DNA

Andrew R. Collins, Catherine Gedik, Sharon Wood, Ann White, Jacques Dubois, Pierre Duez, Jean-François Rees, Rozenn Legall, Liliane Degand, Steffen Loft, …

Free radical research, Vol.36(3), pp.239-245

01/03/2002

DOI: https://doi.org/10.1080/10715760290019246

PMID: 12071341

Abstract

The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CV of less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10 6 Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.

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Title: Inter-laboratory Validation of Procedures for Measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2′-deoxyguanosine in DNA
Creators: Andrew R. Collins - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Catherine Gedik - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Sharon Wood - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Ann White - University of Leicester
Jacques Dubois - Université Libre de Bruxelles
Pierre Duez - Université Libre de Bruxelles
Jean-François Rees - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Rozenn Legall - UCLouvain
Liliane Degand - UCLouvain
Steffen Loft - University of Copenhagen
Annie Jensen - University of Copenhagen
Henrik Poulsen - Copenhagen University Hospital
Allan Weimann - Rigshospitalet
Bente Riis Jensen - Rigshospitalet
Jean Cadet - Commissariat à l'Énergie Atomique et aux Énergies Alternatives
Thierry Douki - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Jean-Luc Ravanat - Commissariat à l'Énergie Atomique et aux Énergies Alternatives
Henry Faure - Centre Hospitalier Universitaire de Grenoble
Michèle Tripier - Centre Hospitalier Universitaire de Grenoble
Isabelle Morel - Université de Rennes
Odile Sergent - Université de Rennes
Pierre Cillard - Université de Rennes
Bénédicte Morin - Université de Bordeaux
Bernd Epe - Johannes Gutenberg University Mainz
Nicole Phoa - Johannes Gutenberg University Mainz
Andrea Hartwig - Karlsruhe University of Education
Anke Pelzer - Karlsruhe University of Education
Piero Dolara - University of Florence
Chiara Casalini - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Francesco Guglielmi - University of Florence
Cristina Luceri - University of Florence
Hiroshi Kasai - University of Occupational and Environmental Health Japan
Rie Kido - University of Occupational and Environmental Health Japan
Ryszard Olinski - Collegium Medicum in Bydgoszcz
Karol Bialkowski - Collegium Medicum in Bydgoszcz
Zdena Dura ková - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Lucia Hlin iková - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Peter Korytar - Comenius University Bratislava
Mária Dušinská - Slovak Medical University
Csilla Mislanová - Slovak Medical University
José Viña - Universitat de València
Ana Lloret - Universitat de València
Lennart Möller - Karolinska Institutet
Tim Hofer - Karolinska Institutet
Eric Gremaud - Nestlé
Laurent Fay - Nestlé
Richard Stadler - Nestlé
Julie Eakins - AstraZeneca
François Pognan - AstraZeneca
John O'Brien - AstraZeneca
Ruan Elliott - Quadram Institute
Siân Astley - Quadram Institute
Angela Bailley - Quadram Institute
Karl Herbert - Commissariat à l'Énergie Atomique et aux Énergies Alternatives
Dina Chauhan - University of Leicester
Frank Kelly - King's College London
Chrisi Dunster - King's College London
Joseph Lunec - 1DNA Instability Laboratory, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
Ian Podmore - University of Leicester
Parul Patel - University of Leicester
Samantha Johnson - University of Leicester
Mark Evans - University of Leicester
Andrea White - University of Leicester
Rex Tyrrell - University of Bath
Matthew Gordon - University of Bath
Chris Wild - University of Leeds
Laura Hardie - University of Leeds
Elizabeth Smith - University of Leeds
ESCODD
Publication Details: Free radical research, Vol.36(3), pp.239-245
Publisher: Informa UK Ltd
Number of pages: 7
Date published: 01/03/2002
Identifiers: 99928704702346
Academic Unit: School of Biosciences
Language: English
Resource Type: Journal article

Inter-laboratory Validation of Procedures for Measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2′-deoxyguanosine in DNA

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