Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

David J Blackbourn; Linda F Chuang; Suganto Sutjipto; Keith F Killam; Paula M McCready; Roy H Doi; Yen Li; Ronald Y Chuang

doi:10.1016/0166-0934(92)90038-F

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Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

Journal article

Peer reviewed

Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

David J Blackbourn, Linda F Chuang, Suganto Sutjipto, Keith F Killam, Paula M McCready, Roy H Doi, Yen Li and Ronald Y Chuang

Journal of virological methods, Vol.37(2), pp.109-117

1992

DOI: https://doi.org/10.1016/0166-0934(92)90038-F

PMID: 1597502

Abstract

RNA

SIV

PCR

A rapid method for the detection of simian immunodeficiency virus (SIV) RNA from peripheral blood mononuclear cells (PBMC) of experimentally infected rhesus macaques by the polymerase chain reaction (PCR) is reported. The PCR was carried out with a complementary DNA (cDNA) template using 3 pairs of primers that were designed to anneal to homologous sequences in conserved regions of 3 molecular clones of SIV mac. The specificity of the primers was confirmed by performing the PCR with template DNA from the 3 molecular clones. SIV-specific RNA was detected from 30 and 50 infected PBMC/6.25 × 10 5 PBMC of two animals.

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Title: Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction
Creators: David J Blackbourn - Department of Medical Pharmacology and Toxicology, School of Medicine, Harvard Medical School, Southborough, MA, U.S.A
Linda F Chuang - Department of Medical Pharmacology and Toxicology, School of Medicine, Harvard Medical School, Southborough, MA, U.S.A
Suganto Sutjipto - California Regional Primate Research Centre, Harvard Medical School, Southborough, MA, U.S.A
Keith F Killam - Department of Medical Pharmacology and Toxicology, School of Medicine, Harvard Medical School, Southborough, MA, U.S.A
Paula M McCready - Department of Biochemistry and Biophysics, University of California, Davis, CA, U.S.A
Roy H Doi - Department of Biochemistry and Biophysics, University of California, Davis, CA, U.S.A
Yen Li - New England Regional Primate Research Centre, Harvard Medical School, Southborough, MA, U.S.A
Ronald Y Chuang - Department of Medical Pharmacology and Toxicology, School of Medicine, Harvard Medical School, Southborough, MA, U.S.A
Publication Details: Journal of virological methods, Vol.37(2), pp.109-117
Publisher: Elsevier B.V
Date published: 1992
Identifiers: 99586585002346
Academic Unit: School of Biosciences
Language: English
Resource Type: Journal article

Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

Abstract

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