Sustaining physical activity may require incorporating activity into everyday routines. Yet, many such routines are executed habitually, so people may not recognise physical activity opportunities. ‘Script Elicitation’ – a novel intervention method whereby participants detail the content and structure of their routines, and are supported to plan modifications to those routines – has not yet been applied to physical activity. This mixed-methods study assessed the acceptability of Script Elicitation for increasing physical activity among office workers. Eleven UK office workers completed the one-to-one Script Elicitation procedure, describing their typical before-, during-, or after-work routines, and receiving guidance on incorporating activity into those routines. One week later, they rated acceptability of the method and completed a semi-structured interview. Physical activity was self-reported at baseline and one-week follow-up. Acceptability was descriptively assessed on two quantitative criteria (no clear decrease in physical activity; above-midpoint acceptability scores) and qualitatively explored via Thematic Analysis. Acceptability criteria were met, and participants reported raised awareness of routines and physical activity increases. Script Elicitation appears potentially suitable for promoting activity within everyday routines. If effectiveness is shown in a more rigorous trial, future work will need to develop script-based methods for efficient delivery at scale as a public health intervention.