Physical activity (PA) has numerous health benefits. However, individuals may depend on it for health reasons or lose control over it to the point of addiction, resulting in adverse health effects. Automatic reactions to cues related to addictive behavior have been identified as a hallmark of addiction. However, the extent to which these automatic reactions are associated with exercise dependence is unknown. This study aimed to examine the associations between exercise dependence and automatic reactions toward PA-related cues. Two studies were conducted on physically active individuals with low-to-moderate levels of exercise dependence. Study 1 (N=65) used a dot-probe task with eye-tracking to examine the association between attentional bias toward PA and exercise dependence scores as measured by the Exercise Dependence Scale–Revised (EDS-R). Study 2 (N=125) used a manikin task and a single-category implicit association test to assess the association of approach-avoidance tendencies and implicit affective attitudes toward PA with exercise dependence scores, respectively. Results showed that exercise dependence scores were positively associated with behavioral indicators of attentional bias (i.e., reaction times), but not with more direct eye-tracking indicators (i.e., first-gaze localization, gaze duration) (Study 1). Similarly, exercise dependence scores were unrelated to approach-avoidance tendencies or implicit affective attitudes toward PA (Study 2). Therefore, our study provides limited evidence supporting the hypothesis that automatic reactions to PA may reflect a “signature” of exercise dependence. Our findings do not robustly support the link between automatic processes and exercise dependence, which raises the question of whether the psychological mechanisms involved in the development of exercise dependence might differ from those typically observed in other addictive behaviors where automatic processes play a significant role. However, due to our sample's low-to-moderate levels of exercise dependence, definitive conclusions cannot be drawn. Further research with individuals exhibiting harmless and addiction-related dependence, incorporating personalized stimuli, and using neurophysiological methods is needed.