Abstract
Twenty years ago, the term ‘Systems Biology’ has been coined, after which Institutes of Systems Biology were established in Tokyo, Seattle and Amsterdam by the pioneers Hiroaki Kitano, Leroy Hood, and Hans Westerhoff, respectively. For their achievements, in 2014 they did receive the recognition of ‘Fellows of The International Society for Systems Biology’. Through their work, and that of their collaborators, the field has progressively matured as an important lever to make biology predictive, testable and quantitative. Systems Biology in combination with Bioinformatics and high-throughput experiments turns data analysis into a forecasting tool. Systems Biology in combination with imaging allows us to understand processes in cells in two or three or even four dimensions.