Abstract
Hepatocyte isolation procedures were developed for tissue derived from Rat, Mouse, Man, Guinea pig, and Hamster. Hepatocyte cultures were derived from the above species, with the exception of Man, and successfully maintained for four days. The cultures were assessed for the ability to metabolise xenobiotics, and exposed to classical inducing agents in vitro. Induction of the cytochrome P-450 monooxygenase system was demonstrated following exposure to phenobarbitone and β-naphthoflavone, and the nature of induction characterised by use of specific enzyme substrates and inhibitors.